Gel-aided sample preparation (GASP) - a simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells

摘要

We describe a "gel-assisted" proteomic sample preparation method for mass spectrometry analysis. Solubilized protein extracts or intact cells are co-polymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation and matrix formation. Gel-aided sample preparation (GASP) has been optimized to be highly flexible, scalable and to allow reproducible sample generation from 50 cells to milligrams of protein extracts. This methodology is fast, sensitive, easy-to-use on a wide range of sample types and accessible to non-specialists.